Review



nikon c2 clsm  (Nikon)


Bioz Verified Symbol Nikon is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Nikon nikon c2 clsm
    Three-dimensional (3D) reconstruction of A. brasilense biofilms by <t>CLSM.</t> Biofilms were stained to visualize some matrix components: red fluorescence indicates extracellular DNA (eDNA) stained with propidium iodide, and blue fluorescence indicates exopolysaccharides stained with Calcofluor. Upper panels: composite 3D images captured at 20x magnification, showing the distribution of both eDNA and EPS. Lower panels: Detailed 3D images of exopolysaccharides captured at 60x magnification. White arrows indicate structures resembling cyst-like cells within the biofilm of the cysK -A mutant strain. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Nikon C2 Clsm, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 60052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+c2+clsm/Objectives/pmc12811640-133-6-9
    Average 99 stars, based on 60052 article reviews
    nikon c2 clsm - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    Three-dimensional (3D) reconstruction of A. brasilense biofilms by CLSM. Biofilms were stained to visualize some matrix components: red fluorescence indicates extracellular DNA (eDNA) stained with propidium iodide, and blue fluorescence indicates exopolysaccharides stained with Calcofluor. Upper panels: composite 3D images captured at 20x magnification, showing the distribution of both eDNA and EPS. Lower panels: Detailed 3D images of exopolysaccharides captured at 60x magnification. White arrows indicate structures resembling cyst-like cells within the biofilm of the cysK -A mutant strain. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Three-dimensional (3D) reconstruction of A. brasilense biofilms by CLSM. Biofilms were stained to visualize some matrix components: red fluorescence indicates extracellular DNA (eDNA) stained with propidium iodide, and blue fluorescence indicates exopolysaccharides stained with Calcofluor. Upper panels: composite 3D images captured at 20x magnification, showing the distribution of both eDNA and EPS. Lower panels: Detailed 3D images of exopolysaccharides captured at 60x magnification. White arrows indicate structures resembling cyst-like cells within the biofilm of the cysK -A mutant strain. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Staining, Fluorescence, Mutagenesis

    A. brasilense AR mutant forms thicker aggregates on radish roots than the wild-type strain. Representative 3D CLSM images of radish roots 7 days post-inoculation. ( A ) Wild-type (WT) A. brasilense Sp7 (harboring pMP2449-5) displays a typical rhizosphere colonization pattern. ( B ) cysK -A mutant ( A. brasilense AR, harboring pMP2449-5) forming visible, thicker, and denser aggregates (white arrows) compared to the WT. For both strains, bacteria are tagged with mCherry (red fluorescence), and the plant root autofluorescence is shown in green. Aggregate thickness was measured from the mCherry channel Z-stacks, revealing a depth of 42.5 μm for the WT and 50 μm for the AR mutant. Images were captured with a 20x objective, 2.0x digital zoom, and steps of 0.5 μm. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: A. brasilense AR mutant forms thicker aggregates on radish roots than the wild-type strain. Representative 3D CLSM images of radish roots 7 days post-inoculation. ( A ) Wild-type (WT) A. brasilense Sp7 (harboring pMP2449-5) displays a typical rhizosphere colonization pattern. ( B ) cysK -A mutant ( A. brasilense AR, harboring pMP2449-5) forming visible, thicker, and denser aggregates (white arrows) compared to the WT. For both strains, bacteria are tagged with mCherry (red fluorescence), and the plant root autofluorescence is shown in green. Aggregate thickness was measured from the mCherry channel Z-stacks, revealing a depth of 42.5 μm for the WT and 50 μm for the AR mutant. Images were captured with a 20x objective, 2.0x digital zoom, and steps of 0.5 μm. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Mutagenesis, Bacteria, Fluorescence

    Related Articles

    Confocal Laser Scanning Microscopy:

    Article Title: ULK1 drives NDP52-mediated selective autophagic degradation of MHC-I to promote immune evasion in HPV-positive head and neck cancer
    Article Snippet: .. Slides were mounted with ProLong Gold AntiFade Mountant (Invitrogen, P36930) and imaged on a Nikon C2 CLSM using NIS-Elements Software. ..

    Article Title: Benzyl isothiocyanate exhibits antibacterial and antibiofilm activity against Fusobacterium nucleatum while preserving viability of human bone marrow mesenchymal stem cells: an in vitro study
    Article Snippet: About 200 μL of working solution (LIVE/DEAD ® BacLight Bacterial Viability kit, Invitrogen, Eugene, OR, USA) was added to each biofilm sample, following the manufacturer's instructions, and then incubated for 30 min at room temperature, protected from light. .. The stained surface was placed face down onto a clean microscope slide and imaged using a Nikon C2 CLSM (Nikon Instruments Inc., Tokyo, Japan) with a 20×/0.75 NA air objective using (λexc) 488 nm/(λem)<550 nm for SYTO ® 9 (BacLightTM Component A) and (λexc) 568 nm/(λem) >600 nm for propidium iodide (BacLightTM Component B). ..

    Article Title: Nonfouling Coatings from Synthetic Intrinsically Disordered Proteins
    Article Snippet: DLS data were analyzed by Zetasizer software version 7.13 (Malvern, U.K.). .. A Nikon C2 CLSM was used to image E. coli on bare gold and B‐M‐E coated transparent gold surfaces. ..

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7
    Article Snippet: .. Confocal images were acquired using a Nikon C2+ CLSM (Nikon, Tokyo, Japan) equipped with a CFI Plan Apo Lambda 20 × objective and two helium–neon lasers for the excitation of the mCherry fluorophore at wavelengths of 540 nm and 650 nm and an argon laser for the excitation of radish autofluorescence at 488 nm. ..

    Article Title: Efficacy of Oxygen Fluid (blue®m) on Human Gingival Fibroblast Viability, Proliferation and Inflammatory Cytokine Expression: An In Vitro Study
    Article Snippet: Samples were finally washed and stored in PBS, and examined under a Nikon C2 confocal laser scanning microscope (CLSM; Nikon Instruments Inc., Tokyo, Japan). .. Sample images were acquired using NIS-Elements Advanced Research software (version 4.0, Nikon, Japan) and Nikon C2 CLSM through 10×/1.84 NA air objective using (λexc) 405 nm/(λem) < 550 nm for DAPI and (λexc) 568 nm/(λem) > 600 nm for Phalloidin [24,25]. .. Cell viability was determined using the AlamarBlueTM viability reagent (Invitrogen, Eugene, OR, USA) according to manufacturer’s instructions.

    Article Title: Efficacy of Oxygen Fluid (blue®m) on Human Gingival Fibroblast Viability, Proliferation and Inflammatory Cytokine Expression: An In Vitro Study
    Article Snippet: The levels of cellular oxidative stress were measured after HGnF cells were stimulated using 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) assay kit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer’s instructions [30]. .. Adherent cells were observed by Nikon C2 CLSM (Nikon Instruments Inc., Tokyo, Japan) with 10×/1.84 NA objective lens using (λexc) 488 nm/(λem) < 550 nm. .. The fields of view were captured randomly and the mean fluorescence intensity (MFI) was quantified using NIS-Elements Advanced Research Software (version 4.0, Nikon, Japan).

    Article Title: Nonfouling Coatings from Synthetic Intrinsically Disordered Proteins.
    Article Snippet: Atomic Force Microscope (AFM): Transparent gold substrates (AU.0100.GC10) and ultra-flat gold substrates (AU.1000.SWTSG) were purchased from Platypus Technologies LLC. .. Confocal Laser Scanning Microscopy (CLSM): A Nikon C2 CLSM was used to image E. coli on bare gold and B-M-E coated transparent gold surfaces. ..

    Software:

    Article Title: ULK1 drives NDP52-mediated selective autophagic degradation of MHC-I to promote immune evasion in HPV-positive head and neck cancer
    Article Snippet: .. Slides were mounted with ProLong Gold AntiFade Mountant (Invitrogen, P36930) and imaged on a Nikon C2 CLSM using NIS-Elements Software. ..

    Article Title: Efficacy of Oxygen Fluid (blue®m) on Human Gingival Fibroblast Viability, Proliferation and Inflammatory Cytokine Expression: An In Vitro Study
    Article Snippet: Samples were finally washed and stored in PBS, and examined under a Nikon C2 confocal laser scanning microscope (CLSM; Nikon Instruments Inc., Tokyo, Japan). .. Sample images were acquired using NIS-Elements Advanced Research software (version 4.0, Nikon, Japan) and Nikon C2 CLSM through 10×/1.84 NA air objective using (λexc) 405 nm/(λem) < 550 nm for DAPI and (λexc) 568 nm/(λem) > 600 nm for Phalloidin [24,25]. .. Cell viability was determined using the AlamarBlueTM viability reagent (Invitrogen, Eugene, OR, USA) according to manufacturer’s instructions.

    Staining:

    Article Title: Benzyl isothiocyanate exhibits antibacterial and antibiofilm activity against Fusobacterium nucleatum while preserving viability of human bone marrow mesenchymal stem cells: an in vitro study
    Article Snippet: About 200 μL of working solution (LIVE/DEAD ® BacLight Bacterial Viability kit, Invitrogen, Eugene, OR, USA) was added to each biofilm sample, following the manufacturer's instructions, and then incubated for 30 min at room temperature, protected from light. .. The stained surface was placed face down onto a clean microscope slide and imaged using a Nikon C2 CLSM (Nikon Instruments Inc., Tokyo, Japan) with a 20×/0.75 NA air objective using (λexc) 488 nm/(λem)<550 nm for SYTO ® 9 (BacLightTM Component A) and (λexc) 568 nm/(λem) >600 nm for propidium iodide (BacLightTM Component B). ..

    Microscopy:

    Article Title: Benzyl isothiocyanate exhibits antibacterial and antibiofilm activity against Fusobacterium nucleatum while preserving viability of human bone marrow mesenchymal stem cells: an in vitro study
    Article Snippet: About 200 μL of working solution (LIVE/DEAD ® BacLight Bacterial Viability kit, Invitrogen, Eugene, OR, USA) was added to each biofilm sample, following the manufacturer's instructions, and then incubated for 30 min at room temperature, protected from light. .. The stained surface was placed face down onto a clean microscope slide and imaged using a Nikon C2 CLSM (Nikon Instruments Inc., Tokyo, Japan) with a 20×/0.75 NA air objective using (λexc) 488 nm/(λem)<550 nm for SYTO ® 9 (BacLightTM Component A) and (λexc) 568 nm/(λem) >600 nm for propidium iodide (BacLightTM Component B). ..

    other:

    Article Title: Electrospun Mats of Fluorosiloxane Copolymers with Antifouling and Antibacterial Performances.
    Article Snippet: The morphology of electrospun mats was observed by SEM (Thermo Fisher Quattro S), while surface roughness was qualitatively assessed using an optical profiler (Contour GT-K 3D).



    Similar Products

    99
    Nikon nikon c2 clsm
    Three-dimensional (3D) reconstruction of A. brasilense biofilms by <t>CLSM.</t> Biofilms were stained to visualize some matrix components: red fluorescence indicates extracellular DNA (eDNA) stained with propidium iodide, and blue fluorescence indicates exopolysaccharides stained with Calcofluor. Upper panels: composite 3D images captured at 20x magnification, showing the distribution of both eDNA and EPS. Lower panels: Detailed 3D images of exopolysaccharides captured at 60x magnification. White arrows indicate structures resembling cyst-like cells within the biofilm of the cysK -A mutant strain. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Nikon C2 Clsm, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+c2+clsm/Objectives/pmc12811640-133-6-9
    Average 99 stars, based on 1 article reviews
    nikon c2 clsm - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    90
    Nikon confocal laser scanning microscope clsm nikon c2/ n-sim
    Three-dimensional (3D) reconstruction of A. brasilense biofilms by <t>CLSM.</t> Biofilms were stained to visualize some matrix components: red fluorescence indicates extracellular DNA (eDNA) stained with propidium iodide, and blue fluorescence indicates exopolysaccharides stained with Calcofluor. Upper panels: composite 3D images captured at 20x magnification, showing the distribution of both eDNA and EPS. Lower panels: Detailed 3D images of exopolysaccharides captured at 60x magnification. White arrows indicate structures resembling cyst-like cells within the biofilm of the cysK -A mutant strain. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Confocal Laser Scanning Microscope Clsm Nikon C2/ N Sim, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+c2+clsm/pm40506300-56-27-32
    Average 90 stars, based on 1 article reviews
    confocal laser scanning microscope clsm nikon c2/ n-sim - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    Three-dimensional (3D) reconstruction of A. brasilense biofilms by CLSM. Biofilms were stained to visualize some matrix components: red fluorescence indicates extracellular DNA (eDNA) stained with propidium iodide, and blue fluorescence indicates exopolysaccharides stained with Calcofluor. Upper panels: composite 3D images captured at 20x magnification, showing the distribution of both eDNA and EPS. Lower panels: Detailed 3D images of exopolysaccharides captured at 60x magnification. White arrows indicate structures resembling cyst-like cells within the biofilm of the cysK -A mutant strain. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Three-dimensional (3D) reconstruction of A. brasilense biofilms by CLSM. Biofilms were stained to visualize some matrix components: red fluorescence indicates extracellular DNA (eDNA) stained with propidium iodide, and blue fluorescence indicates exopolysaccharides stained with Calcofluor. Upper panels: composite 3D images captured at 20x magnification, showing the distribution of both eDNA and EPS. Lower panels: Detailed 3D images of exopolysaccharides captured at 60x magnification. White arrows indicate structures resembling cyst-like cells within the biofilm of the cysK -A mutant strain. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Confocal images were acquired using a Nikon C2+ CLSM (Nikon, Tokyo, Japan) equipped with a CFI Plan Apo Lambda 20 × objective and two helium–neon lasers for the excitation of the mCherry fluorophore at wavelengths of 540 nm and 650 nm and an argon laser for the excitation of radish autofluorescence at 488 nm.

    Techniques: Staining, Fluorescence, Mutagenesis

    A. brasilense AR mutant forms thicker aggregates on radish roots than the wild-type strain. Representative 3D CLSM images of radish roots 7 days post-inoculation. ( A ) Wild-type (WT) A. brasilense Sp7 (harboring pMP2449-5) displays a typical rhizosphere colonization pattern. ( B ) cysK -A mutant ( A. brasilense AR, harboring pMP2449-5) forming visible, thicker, and denser aggregates (white arrows) compared to the WT. For both strains, bacteria are tagged with mCherry (red fluorescence), and the plant root autofluorescence is shown in green. Aggregate thickness was measured from the mCherry channel Z-stacks, revealing a depth of 42.5 μm for the WT and 50 μm for the AR mutant. Images were captured with a 20x objective, 2.0x digital zoom, and steps of 0.5 μm. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant forms thicker aggregates on radish roots than the wild-type strain. Representative 3D CLSM images of radish roots 7 days post-inoculation. ( A ) Wild-type (WT) A. brasilense Sp7 (harboring pMP2449-5) displays a typical rhizosphere colonization pattern. ( B ) cysK -A mutant ( A. brasilense AR, harboring pMP2449-5) forming visible, thicker, and denser aggregates (white arrows) compared to the WT. For both strains, bacteria are tagged with mCherry (red fluorescence), and the plant root autofluorescence is shown in green. Aggregate thickness was measured from the mCherry channel Z-stacks, revealing a depth of 42.5 μm for the WT and 50 μm for the AR mutant. Images were captured with a 20x objective, 2.0x digital zoom, and steps of 0.5 μm. Scale bar = 25 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Confocal images were acquired using a Nikon C2+ CLSM (Nikon, Tokyo, Japan) equipped with a CFI Plan Apo Lambda 20 × objective and two helium–neon lasers for the excitation of the mCherry fluorophore at wavelengths of 540 nm and 650 nm and an argon laser for the excitation of radish autofluorescence at 488 nm.

    Techniques: Mutagenesis, Bacteria, Fluorescence